Consulting

Expectations from Statistical Consulting/Analysis of Microarray Experiments

Cancer Stem Cell Network

Statistical consulting for experiments in the Cancer Stem Cell Network is available by contacting Biostatistician Changjiang Xu at changjiang.xu@utoronto.ca

Office hours : Tuesday 3:00pm-5:00pm and Thursday 11:00am -1:00pm.

In order to ensure that appropriate advice be given, the consulting is strongly suggested at the design stage of the experiment.

Upfront required Information from researcher

· Please provide a complete and concise summary of information about the experiment,

· Experimental units i.e., animal strain/Humans, etc. How they were selected?

· Sample preparation procedure if any?

· If experiment conducted on different days/times etc?

· Clear and concise research questions that you want to investigate

· Specify the number of experimental conditions (factors) that you plan to study, that might influence the response variable (gene expression values in case of microarray experiments)

· Number of stages that the experiment will be conducted. This will help us make sure that any factor (condition) of interest is not confounded with some non-biological factor, etc?

· The type of analysis that the researcher would think would be suitable to perform

· What type of chip would be used, if microarray experiment?

· How many samples that researcher is planning based on budget

After this upfront meeting, the statistician would take a week to make a framework for the issues and suggest the statistical feasibility for the project etc. The statistician would give recommendations for the number of samples, any other design issues, randomization, blocking etc.

Downloads

• Report on Set XX Word DOC

• Cytoscape Session file "" (fig.3) CYS

• Enrichment Map "" (fig.3) PDF PNG