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← Revision 17 as of 2014-06-26 20:24:16 ⇥
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| Statistical data analysis for experiments in the Cancer Stem Cell Program is available by contacting Biostatistician Changjiang Xu at changjiang.xu@utoronto.ca. In order to ensure that appropriate analyses can be performed and all relevant information is provided, the following guidelines must be followed. 1. '''Required Laboratory Information''': a complete and concise information about the experiment, including the following |
Statistical data analysis for experiments in the Cancer Stem Cell Program is available by contacting Biostatistician Changjiang Xu at changjiang.xu@utoronto.ca. The following guidelines would be helpful to ensure that all relevant information is provided. 1. '''Laboratory Information''': |
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| * Researcher (student/postdoc) associated and contact information | * Researcher associated and contact information |
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| * If different samples were analyzed on different days, a list of which samples were analyzed on which days is needed. * The experimental layout of the study, i.e. which samples are located where on the chip results (tracking worksheet for the experiment) [if relevant] |
* a list of which samples were analyzed on which days if different samples were analyzed on different days * The experimental layout of the study, i.e. which samples are located where on the chip results |
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| 2. '''Raw data files''': standard format (comma delimited .csv format. In case of affymetrix .CEL files and CDF file to decode each .CEL file) * No comment fields in the data * Exactly one header line, naming all the columns using variable names that do not contain any spaces or underscores * A relevant set of row labels (e.g. probe_IDs) |
2. '''Raw data files''': * Standard format |
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| 3. '''Description of the experiment’s goals''': clearly stated, including primary and secondary objectives, etc., proposed plan of analysis, if biologists have any particular technique in mind, and the biological rationale behind that choice. Otherwise the biostatistician will choose the type of analysis after discussing with researcher. | 3. '''Description of the experiment’s goals''': * Primary and secondary objectives * Proposed plan of analysis if biologists have any particular technique in mind |
Data Requirements
Statistical data analysis for experiments in the Cancer Stem Cell Program is available by contacting Biostatistician Changjiang Xu at changjiang.xu@utoronto.ca. The following guidelines would be helpful to ensure that all relevant information is provided.
Laboratory Information:
- PI
- Researcher associated and contact information
- Technician
- Animal strain (i.e. A/J mice)
- Tissue/sample (i.e. lymphocytes)
- Data type (i.e. gene expression data)
- Chip used, along with version
- Day/Date when the experiment was conducted,
- a list of which samples were analyzed on which days if different samples were analyzed on different days
- The experimental layout of the study, i.e. which samples are located where on the chip results
- Explanations for the column labels used in the raw data files
- Copy of Tracking worksheet for your experiment
Raw data files:
- Standard format
- Annotation file if your lab has prepared any special file
Description of the experiment’s goals:
- Primary and secondary objectives
- Proposed plan of analysis if biologists have any particular technique in mind