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Describe CancerStemCellProject/VeroniqueVoisin/PathwayAnalysisService/Tutorials here. | #acl All:read = Online tutorials = All the software used are freely available (open-source) and easy to install on your computer. === Gene-Set Enrichment Analysis (GSEA) === *http://www.broadinstitute.org/gsea/index.jsp : Gene Set Enrichment Analysis (GSEA) is a computational method that determines whether an a priori defined set of genes shows statistically significant, concordant differences between two biological states (e.g. phenotypes). We use GSEA at the first analysis step. As we will perform this analysis for you, you don't need to download GSEA. === Cytoscape === * http://www.cytoscape.org/: Cytoscape is an open source bioinformatics software platform for visualizing molecular interaction networks and biological pathways and integrating these networks with annotations, gene expression profiles and other state data Download Cytoscape 2.8.0 from http://www.cytoscape.org/download.html (need to enter, name, institution, e-mail address but no account necessary) Cytoscape tutorial: http://cytoscape.wodaklab.org/wiki/Presentations/Basic === EnrichmentMap === * http://baderlab.org/EnrichmentMap Enrichment Map is a visualization method for gene set enrichment results which helps quickly find general functional themes in genomics data. Enrichment Map works as a plug-in for Cytoscape. To install it, download the zipped file, move it to the Cytoscape plugin directory and unzip it. === WordCloud === * http://baderlab.org/WordCloud WordCloud is a Cytsocape plugin that generates a word tag cloud from a user-defined node selection, summarizing an attribute of choice. It notably eases the interpretation of the Enrichment Map. Download the WordCloud plugin and put the file in the Cytoscape plugin directory, unzip it and put the WordCloud.jar file in the plugin directory. === GeneMANIA === *http://genemania.org GeneMANIA is a free public resource that offers a simple, intuitive web interface that shows the relationships between genes in a list and analyzes and extends the list to include other related genes. You can use GeneMANIA to find new members of a pathway or complex, find additional genes you may have missed in your screen or find new genes with a specific function. You also can use GeneMANIA as a Cytoscape plugin. You can find the GeneMANIA tutorial at: http://genemania.org/pages/help.jsf== How to explore an interactive Enrichment Map on your computer == = How to explore an interactive Enrichment Map on your computer = === Download the sofware you need (see below for download and tutorial information) === 1. Cytoscape * Enrichment Map plugin * WordCLoud plugin === Explore the Enrichment map using the .cys file that we give you === * Download the .cys file * Put the .cys file in the directory of your choice * In Cytoscape, go to Open, File and browse the directories to locate your file and click Open. *Explore the map * The “Parameters” tab in the “Results Panel” on the right side of the window contains a legend mapping the colours to the phenotypes and displaying the parameters used to create the map (cut-off values and data files). * The “Network” tab in the “Control Panel” on the left lists all available networks in the current session and at the bottom has a overview of the current network which allows to easily navigate in a network even at higher zoom levels by dragging the blue rectangle (the current view) over the network. *Clicking on a node (the circle that represents a gene set) will open the “EM Geneset Expression Viewer” tab in the “Data Panel” showing a heatmap of the expression values of all genes in the selected gene set. *Clicking on an edge (the line between two nodes) will open the “EM Overlap Expression Viewer” tab in the “Data Panel” showing a heatmap of the expression values of all genes both gene sets that are connected by this edge have in common. *If several nodes and edges are selected (e.g. by dragging a selection box around the desired gene sets) the “EM Geneset Expression Viewer” will show the union of all genes in the selected gene sets and the “EM Overlap Expression Viewer” will show only those genes that all selected gene sets have in common. *The “Geneset Summary” tab in the “Results Panel” on the right contains information about which nodes and edges are selected. === Tips === * Click on “View / Show Graphics Details” to see the map details even on low zoom-levels ---- ----- [[CancerStemCellProject/VeroniqueVoisin/PathwayAnalysisService/SOP | BACK TO SOP ]] [[CancerStemCellProject/VeroniqueVoisin/PathwayAnalysisService | BACK TO HOME PAGE ]] |
Online tutorials
All the software used are freely available (open-source) and easy to install on your computer.
Gene-Set Enrichment Analysis (GSEA)
http://www.broadinstitute.org/gsea/index.jsp :
- Gene Set Enrichment Analysis (GSEA) is a computational method that determines whether an a priori defined set of genes shows statistically significant, concordant differences between two biological states (e.g. phenotypes). We use GSEA at the first analysis step. As we will perform this analysis for you, you don't need to download GSEA.
Cytoscape
- Cytoscape is an open source bioinformatics software platform for visualizing molecular interaction networks and biological pathways and integrating these networks with annotations, gene expression profiles and other state data
Download Cytoscape 2.8.0 from http://www.cytoscape.org/download.html (need to enter, name, institution, e-mail address but no account necessary) Cytoscape tutorial: http://cytoscape.wodaklab.org/wiki/Presentations/Basic
- Cytoscape is an open source bioinformatics software platform for visualizing molecular interaction networks and biological pathways and integrating these networks with annotations, gene expression profiles and other state data
EnrichmentMap
http://baderlab.org/EnrichmentMap
- Enrichment Map is a visualization method for gene set enrichment results which helps quickly find general functional themes in genomics data. Enrichment Map works as a plug-in for Cytoscape. To install it, download the zipped file, move it to the Cytoscape plugin directory and unzip it.
WordCloud
WordCloud is a Cytsocape plugin that generates a word tag cloud from a user-defined node selection, summarizing an attribute of choice. It notably eases the interpretation of the Enrichment Map. Download the WordCloud plugin and put the file in the Cytoscape plugin directory, unzip it and put the WordCloud.jar file in the plugin directory.
GeneMANIA
GeneMANIA is a free public resource that offers a simple, intuitive web interface that shows the relationships between genes in a list and analyzes and extends the list to include other related genes. You can use GeneMANIA to find new members of a pathway or complex, find additional genes you may have missed in your screen or find new genes with a specific function. You also can use GeneMANIA as a Cytoscape plugin. You can find the GeneMANIA tutorial at: http://genemania.org/pages/help.jsf== How to explore an interactive Enrichment Map on your computer ==
How to explore an interactive Enrichment Map on your computer
Download the sofware you need (see below for download and tutorial information)
- Cytoscape
- Enrichment Map plugin
- WordCLoud plugin
Explore the Enrichment map using the .cys file that we give you
- Download the .cys file
- Put the .cys file in the directory of your choice
- In Cytoscape, go to Open, File and browse the directories to locate your file and click Open.
- Explore the map
- The “Parameters” tab in the “Results Panel” on the right side of the window contains a legend mapping the colours to the phenotypes and displaying the parameters used to create the map (cut-off values and data files).
- The “Network” tab in the “Control Panel” on the left lists all available networks in the current session and at the bottom has a overview of the current network which allows to easily navigate in a network even at higher zoom levels by dragging the blue rectangle (the current view) over the network.
- Clicking on a node (the circle that represents a gene set) will open the “EM Geneset Expression Viewer” tab in the “Data Panel” showing a heatmap of the expression values of all genes in the selected gene set.
- Clicking on an edge (the line between two nodes) will open the “EM Overlap Expression Viewer” tab in the “Data Panel” showing a heatmap of the expression values of all genes both gene sets that are connected by this edge have in common.
- If several nodes and edges are selected (e.g. by dragging a selection box around the desired gene sets) the “EM Geneset Expression Viewer” will show the union of all genes in the selected gene sets and the “EM Overlap Expression Viewer” will show only those genes that all selected gene sets have in common.
- The “Geneset Summary” tab in the “Results Panel” on the right contains information about which nodes and edges are selected.
Tips
- Click on “View / Show Graphics Details” to see the map details even on low zoom-levels