Enrichment Map Gene Sets

EnrichmentMap is a Cytoscape plugin developed in the Baderlab to help visualize, navigate and analyze functional enrichment results as generated from programs such as Gene Set Enrichment Analysis(GSEA), BiNGO, or David. Some enrichment programs, such as GSEA, allow the user to search against their own gene set database. As annotation (gene set) sources are regularly updated as new information is discovered we set up an automated system to update our gene set collections so we are always using the most up-to-date annotations.

If you use these gene sets, please cite our Enrichment Map paper.

Important Note (January 2016) - with the latest build of pathways we have removed KEGG from the main compilation set of pathways. If you would like to include KEGG in your analysis the sets are located in the misc/ directory and can be appended to your gmt file.

Important Note (April 2012) - Genesets files from December 2011, January 2012, Februrary 2012, and March 2012 had an error in the up-propagation of GO. Up-propagation only followed the is-a relationship and did not follow the part-of relationship which translates into missing annotations. This primarily effects genesets in GO cellular compartment.

Summary

• Gene Set Files can be downloaded from : http://download.baderlab.org/EM_Genesets/

• Enrichment Map Gene Sets are a set of Gene Set files in GMT format (compatible with GSEA) updated monthly from original source locations available with:

  1. Entrez gene ids

  2. UniProt accessions

  3. Gene symbols
• The GMT File format contains one Gene Set per line. Each line contains:
  • Name (tab) Description (tab) Gene (tab) Gene (tab) ...
  • In our format:
    • Name = Gene Set Name % Gene Set Source % Gene Set Source identifier

      • Example --> ATP-dependent protein binding%GO%GO:0043008 OR arginine biosynthesis IV%HUMANCYC%ARGININE-SYN4-PWY

    • Description = Gene Set Name

      • Example --> ATP-dependent protein binding OR arginine biosynthesis IV

    • Gene = identified by one of the three possible identifiers (Entrez gene id, UniProt accession or gene symbols)

    • IMPORTANT NOTE: Originally we used the "|" to separate information in the Name field but we came across issues with this separator in GSEA so we changed to "%". The "%" was used as of the December 2011 build.

• In the main directory (current_release/Human/symbol) there are 5 primary files to choose from:

  1. Human_GO_AllPathways_with_GO_iea_{Date}_{ID}.gmt – contains genesets from all 3 divisions of GO (biological process, molecular function, cellular component) including annotations that have evidence code IEA (inferred from electronic annotation), ND (no biological data available), and RCA (inferred from reviewed computational analysis) and all pathway resources.

  2. Human_GO_AllPathways_no_GO_iea_{Date}_{ID}.gmt – contains genesets from all 3 divisions of GO (biological process, molecular function, cellular component) excluding annotations that have evidence code IEA (inferred from electronic annotation), ND (no biological data available), and RCA (inferred from reviewed computational analysis) and all pathway resources.

  3. Human_GOBP_AllPathways_with_GO_iea_{Date}_{ID}.gmt – contains only genesets from GO biological process including annotations that have evidence code IEA (inferred from electronic annotation), ND (no biological data available), and RCA (inferred from reviewed computational analysis) and all pathway resources.

  4. Human_GOBP_AllPathways_no_GO_iea_{Date}_{ID}.gmt – (recommended file) contains only genesets from GO biological process excluding annotations that have evidence code IEA (inferred from electronic annotation), ND (no biological data available), and RCA (inferred from reviewed computational analysis) and all pathway resources.

  5. Human_AllPathways_{Date}_{ID}.gmt – contains only genesets from all pathways resources.

Current Stats

• Human - http://download.baderlab.org/EM_Genesets/current_release/Human/Summary_Geneset_Counts.txt

• Mouse - http://download.baderlab.org/EM_Genesets/current_release/Mouse/Summary_Geneset_Counts.txt

Sources

• Human

• Mouse

Specialty Gene Sets

• The bulk of our genesets are groupings from similar biological processes, pathways and functional annotations but there are a few additional collections of sets that we don't group with them. They include:

  1. miRs - sets consisting of all the targets for a given microRNA.

     • miR genesets are retrieved from Msigdb c3 collection.

  2. Transcription Factors - sets consisting of all the targets for a given transcription factor.

     • TF genesets are retrieved from Msigdb c3 collection.

  3. Disease Phenotype - sets consisting of all known proteins associated with the given disease.

     • Disease phenotype genesets are retrieved from the Human phenotype ontology. Genes associated with a particular disease are annotated to it. In addition, in the same style as the Gene Ontology, the relationship between each disease is stored creating an ontology of diseases. Annotations are up-propagated to related disease terms.

  4. Drugs Targets - sets consisting of all the known or predicted targets for a given drug.

     • Drug target information is retrieved from drugbank. Drugbank is a resource containing 6711 drug entries including 1447 FDA-approved small molecule drugs, 131 FDA-approved biotech (protein/peptide) drugs, 85 nutraceuticals and 5080 experimental drugs. In addition to the compilation of all drugs contained in drugbank geneset files are also created for each of the defined drug categories including approved, experimental, illicit, nutraceutical, and small molecule.

File Structure

< > denotes directory

• <Release> - directory is named according to date sets were updated.

  • <Species>

    • <Identifier> - (either Entrez gene, UniProt, Gene symbol)

      • <GO>

        • BP = biological process
        • MF = molecular function
        • CC = Cellular component
        • All = BP + MF + CC

        • no_GO_IEA - indicates that the file excludes GO annotations with evidence codes - 'IEA' (inferred from electronic annotation), 'ND' (No biological data available), 'RCA' (inferred from reviewed computational analysis)

        • with_GO_IEA - indicates that the file includes GO annotations with evidence codes - 'IEA' (inferred from electronic annotation), 'ND' (No biological data available), 'RCA' (inferred from reviewed computational analysis)

      • <Pathways>

      • <miRs>

      • <TF>

      • <Disease phenotypes>

• In each <identifier> directory There are amalgamated Gene Set files:

  • AllPathways - contains all pathway sources in the Pathways directory

  • GOPathways - contains all GO (MF, BP, CC) and all Pathway sources in the Pathways directory.

Creating customized Gene Sets

1. Download the desired gene set files you would like to use in your customized set and concatenate the files.
   For example, to combine Human_IOB_Entrezgene.gmt Human_NetPath_Entrezgene.gmt, you can use the following linux command:

   cat Human_IOB_Entrezgene.gmt Human_NetPath_Entrezgene.gmt > MyCustomizedSet.gmt

References

1. Kanehisa M, Goto S, Sato Y, Furumichi M, Tanabe M. KEGG for integration and interpretation of large-scale molecular data sets. Nucleic Acids Res. 2011 Nov 10. PMID: 22080510
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2. Subramanian A, Tamayo P, Mootha VK, Mukherjee S, Ebert BL, Gillette MA, Paulovich A, Pomeroy SL, Golub TR, Lander ES, Mesirov JP. Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles. Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50. PMID: 16199517
   Pubmed

3. Schaefer CF, Anthony K, Krupa S, Buchoff J, Day M, Hannay T, Buetow KH. PID: the Pathway Interaction Database. Nucleic Acids Res. 2009 Jan;37(Database issue):D674-9. PMID: 18832364
   Pubmed

4. Kandasamy K, et a NetPath: a public resource of curated signal transduction pathways.Genome Biol. 2010 Jan 12;11(1):R3. PMID: 20067622
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5. Romero P, Wagg J, Green ML, Kaiser D, Krummenacker M, Karp PD. Computational prediction of human metabolic pathways from the complete human genome. Genome Biol. 2005;6(1):R2. Epub 2004 Dec 22. PMID: 15642094
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7. Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, Davis AP, Dolinski K, Dwight SS, Eppig JT, Harris MA, Hill DP, Issel-Tarver L, Kasarskis A, Lewis S, Matese JC, Richardson JE, Ringwald M, Rubin GM, Sherlock G. Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet. 2000 May;25(1):25-9. PMID: 10802651
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8. Mi H, Lazareva-Ulitsky B, Loo R, Kejariwal A, Vandergriff J, Rabkin S, Guo N, Muruganujan A, Doremieux O, Campbell MJ, Kitano H, Thomas PD. The PANTHER database of protein families, subfamilies, functions and pathways. Nucleic Acids Res. 2005 Jan 1;33(Database issue):D284-8. PubMed PMID: 15608197
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