Enrichment Map Genesets

Contents

Enrichment Map Genesets

Summary

Enrichment Map Genesets are a set of Gene Set files in GMT format (compatible with GSEA) updated monthly from original source locations available with:
1. Entrez gene ids
2. UniProt accessions
3. Gene symbols

Sources

Human

Source	File Origin	File Type	ID extracted	Frequency source is updated	Number of pathwayss	Notes
KEGG	KEGG ftp site (July 2011)	gmt	symbol	static as of July 1, 2011	236	Not available in biopax, available in flatfile, translated into gmt files
Msigdb - c2	static (needs to be updated manually)	gmt	Entrez gene	sporadically	Biocarta - 217 Other - 47	Only need other and Biocarta as all other sources are currently covered
NCI	NCI	biopax	Entrez gene	sporadically	219 pathways
Biocarta	NCI	biopax	Entrez gene	static	386 pathways	Biopax 3 - Complete Mess! - currently getting from Msigdb
IOB	directly from IOB - static (July 2011)	biopax	Entrez gene	sporadically	35 pathways - 10 are the same as CellMap, 1 is the same as NetPath	need biopax pathways fixed so species info is correct but information is still extractable.
NetPath	www.netpath.org/browse (scripted grab of file numbered 1-25)	biopax	Entrez gene	static	25 pathways - 12 are cancer pathways (10 are CellMap) 13 are immunity pathways	need biopax pathways fixed so species info is correct but information is still extractable.
HumanCyc	scripted grab of zipped release from password protected website.	biopax	Uniprot	updated periodically	249 Pathways	available in biopax level 2 and level 3
Reactome	scripted grab of zipped release from website	biopax	Uniprot	updated release	1117 pathways (release 37)	No way of getting version of release from biopax file
GO	scripted grab from EBI ftp site (human)	GAF	Uniprot	released once a month	13,034 no GO IEA 15,181 with GO IEA	source is direct from original curator of annotations
msigdb - c3 Specialty GMTs mirs, transcription factors	grab from Msigdb	gmt	Entrez gene	sporadically	221 miRs 616 TFs

Mouse

Source	File Origin	File Type	ID extracted	Frequency source is updated	Number of pathwayss	Notes
Reactome	scripted grab of zipped release from website	biopax	Uniprot	updated release	946 pathways (release 37)	No way of getting version of release from biopax file
GO	scripted grab from MGI ftp site (human)	GAF	MGI	released once a month	14,563 no GO IEA 15,041 with GO IEA	source is direct from original curator of annotations
KEGG	translated from Human using Homologene	gmt	Entrezgene	static as of July 1, 2011	236	Not available in mouse specific format
Msigdb - c2	translated from Human using Homologene	gmt	Entrez gene	sporadically	total 880: Kegg -186 Reactome - 430 Biocarta - 217 Other - 47	Only need other and Biocarta as all other sources are currently covered
NCI	translated from Human using Homologene	gmt	Entrez gene	sporadically	219 pathways
IOB	translated from Human using Homologene	gmt	Entrez gene	sporadically	35 pathways - 10 are the same as CellMap, 1 is the same as NetPath	need biopax pathways fixed so species info is correct but information is still extractable.
NetPath	translated from Human using Homologene	gmt	Entrez gene	static	25 pathways - 12 are cancer pathways (10 are CellMap) 13 are immunity pathways	need biopax pathways fixed so species info is correct but information is still extractable.
HumanCyc	translated from Human using Homologene	gmt	Entrez gene	updated periodically	249 Pathways	available as Mousecyc in biopax but when we parsed it we got a fraction of the pathways that are in human so chose to convert the human files instead

File Structure

< > denotes directory

<Release> - directory is named according to date sets were updated.
- <Species>
  - <Identifier> - (either Entrez gene, UniProt, Gene symbol)
    - <GO>
      - BP = biological process
      - MF = molecular function
      - CC = Cellular component
      - All = BP + MF + CC
      - no_GO_IEA - indicates that the file excludes GO annotations with evidence codes - 'IEA' (inferred from electronic annotation), 'ND' (No biological data available), 'RCA' (inferred from reviewed computational analysis)
      - with_GO_IEA - indicates that the file includes GO annotations with evidence codes - 'IEA' (inferred from electronic annotation), 'ND' (No biological data available), 'RCA' (inferred from reviewed computational analysis)
    - <Pathways>
    - <miRs>
    - <TF>
    - <Disease phenotypes>
In each <identifier> directory There are amalgamated gene set files:
- AllPathways - contains all pathway sources in the Pathways directory
- GOPathways - contains all GO (mf, bp, cc) and all Pathway sources in the Pathways directory.

Creating customized Genesets

Download the desired gene set files you would like to use in your customized set. (For example Human_IOB_Entrezgene.gmt Human_NetPath_Entrezgene.gmt )

   cat Human_IOB_Entrezgene.gmt Human_NetPath_Entrezgene.gmt > MyCustomizedSet.gmt