Enrichment Map User Guide

Overview

The Enrichment Map Cytoscape Plugin allows you to visualize the results of gene-set enrichment as a network. It will operate on any generic enrichment results as well as specifically on Gene Set Enrichment Analysis (GSEA) results. Nodes represent gene-sets and edges represent mutual overlap; in this way, highly redundant gene-sets are grouped together as clusters, dramatically improving the capability to navigate and interpret enrichment results.

Gene-set enrichment is a data analysis technique taking as input

1. a (ranked) gene list, from a genomic experiment
   

2. gene-sets, grouping genes on the basis of a-priori knowledge (e.g. Gene Ontology) or experimental data (e.g. co-expression modules)

and generating as output the list of enriched gene-sets, i.e. best sets that summarizing the gene-list. It is common to refer to gene-set enrichment as functional enrichment because functional categories (e.g. Gene Ontology) are commonly used as gene-sets.

---

Installation

The Enrichment Map Plugin requires Cytoscape Version 2.6.x. If you don't have Cytoscape or an older Version (2.5 or older), please download the latest Release from http://www.cytoscape.org/ and install it on your computer.

• Download the Enrichment Map plugin from Software/EnrichmentMap and manually place the file EnrichmentMap.jar in the Cytoscape/plugins folder.

---

Quick Start Guide

Creating an Enrichment Map

You have a few different options:

• Load GSEA Results
• Load Generic Results
• Load David Results
• Load Bingo Results

The only difference between the above modes is the structure of the enrichment table(s). In either case, to use the plugin you will need the following files:

• file.gmt: gene-set to gene ID
• file.txt or .gct: expression matrix [OPTIONAL]
• file.txt or .xls (*): enrichment table(s)

(*) GSEA saves the enrichment table as a .xls file; however, these are not true Excel files, they are tab-separated text files with a modified extension; Enrichment Map does not work with "true" Excel .xls files.

If your enrichment results were generated from GSEA, you will just have to pick the right files from your results folder. If you have generated the enrichment results using another method, you will have to go to the Full User Guide, File Format section, and make sure that the file format complies with Enrichment Map requirements.

You can use the parameter defaults. For a more careful choice of the parameter settings, please go to the Full User Guide, Tips on Parameter Choice.

Graphical Mapping of Enrichment

• Nodes represent gene-sets.
• Edges represent mutual overlap.
• Enrichment significance (p-value) is conveyed as node colour intensity.

• The enriched phenotype is conveyed by node colour hue.
  

  • Note: In standard two-class designs, where two phenotypes are compared (e.g. treated vs untreated) the colour hue conveys the enriched phenotype; this is equivalent to mapping enrichment in up- and down-regulated genes, if one of the two phenotypes is assumed as reference (e.g. untreated), and the other phenotype is the one of interest; in such a case, enriched in the phenotype of interest means up, and enrichment in the reference phenotype means down.

• Node size represents how many genes are in the gene-set.

Exploring the Enrichment Map

• The "Parameters" tab in the "Results Panel" on the right side of the window contains a legend mapping the colours to the phenotypes and displaying the parameters used to create the map (cut-off values and data files).
• The "Network" tab in the "Control Panel" on the left lists all available networks in the current session and at the bottom has a overview of the current network which allows to easily navigate in a network even at higher zoom levels by dragging the blue rectangle (the current view) over the network.
• Clicking on a node (the circle that represents a gene set) will open the "EM Geneset Expression Viewer" tab in the "Data Panel" showing a heatmap of the expression values of all genes in the selected gene set.
• Clicking on an edge (the line between two nodes) will open the "EM Overlap Expression Viewer" tab in the "Data Panel" showing a heatmap of the expression values of all genes both gene sets that are connected by this edge have in common.
• If several nodes and edges are selected (e.g. by dragging a selection box around the desired gene sets) the "EM Geneset Expression Viewer" will show the union of all genes in the selected gene sets and the "EM Overlap Expression Viewer" will show only those genes that all selected gene sets have in common.

Advanced tips

• With large networks and low zoom-levels Cytoscape automatically reduces the details (such as hiding the node labels and not showing the node borders). To override this mechanism click on "View / Show Graphics Details"

• The VizMapper and the Node- and Edge Attribute Browser open up a lot more visualization options like linking the label size to Enrichment Scores or p-values. Refer to the Cytoscape manual at www.cytoscape.org for more information.

• If you have used Genesets from GSEAs MSigDb, you can access additional informations for each gene set, by adding the a new property:
  

  • (Edit / Preferences / Properties... / Add -> enter property name: nodelinkouturl.MSigDb -> enter property value: http://www.broad.mit.edu/gsea/msigdb/cards/%ID%.html -> [ ] Make Current Cytoscape Properties default -> (OK) ). Now you can right-click on a node and choose LinkOut/MSigDb to open the Database entry of the Geneset represented by that node in your Browser.

• When loading GSEA results there is no need to specify each file. Use the GSEA RPT file to auto-populate all the file fields in the EM interface. Check out: How to use RPT files

• You can specify more lax p-value, q-value and coefficient threshold initially and fine tune them after the network is created by adjusting them through the p-value, q-value and coefficient tuners in the results panel. Check out:How to use Parameters Panel

---

Full User Guide

File Formats

Gene sets file (GMT file)

• Each row of the geneset file represents one geneset and consists of:
  • geneset name (--tab--) description (--tab--) a list of tab-delimited genes that are part of that geneset.
• The geneset names must be unique.
• The gene set file describes the genesets used for the analysis. These files can be obtained

  1. directly downloading our monthly updated gene-set collections from Baderlab geneses collections. Description of sources and methods used to create collection can be found here

  2. directly downloading gene-sets collected in the MSigDB
     

  • Note: if you use MSigDB Gene Ontology gene-sets, please consider that they do not include all annotations, as an evidence code filter is applied; if you are interested in achieving maximum coverage, download the original annotations

    1. converting gene annotations / pathways from public databases
       

  • ~-Note: if you are a R user, Bioconductor offers annotation packages such as GO.db, org.Hs.eg.db, KEGG.db

Expression Data file (GCT, TXT or RNK file) [OPTIONAL]

• The expression data can be loaded in three different formats: gct (GSEA file type), rnk (GSEA file type) or txt.
• The expression data serves two purposes:
  • Expression data is used by the Heatmap when clicking on nodes and edges in the Enrichment map so the expression of subsets of data can be viewed.
  • Gene sets are filtered based on the genes present in the expression file. For example, if Geneset X contains genes {1,2,3,4,5} but the expression file only contain expression value for genes {1,2,3} Geneset X will be represented as {1,2,3} in the Enrichment Map.
• Expression data is not required. In the absence of an expression file Enrichment map will create a dummy expression file to associate with the data set. The dummy expression gives an expression value of 1 for all the genes associated with the enriched genesets in the Enrichment map.

• [Note: if you are running a two dataset analysis with no expression files the genes for each dataset is calculated based on the enriched genesets. If a geneset is enriched in one dataset and not the other this could create different subsets of genes associated to each datasets and create multiple edges between genesets. To avoid this, create a fake expression file with the set of genes used for both analyses.]

GCT (GSEA file type)

• GCT differs from TXT only because of two additional lines that are required at the top of the file.

• The GCT file contains two additional lines at the top of the file.
  • The first line contains #1.2.
  • The second line contains the number of data rows (tab) the number of data columns.
  • The third line consists of column headings.
    • name (--tab--) description (--tab--) sample1 name (--tab--) sample2 name …
  • Each line of expression file contains a:
    • name (--tab--) description (--tab--) followed by a list of tab delimited expression values.
• NOTE: If the GCT file contains Probeset ID's as primary keys (e.g. as you had GSEA collapse your data file to gene symbols) you need to convert the gct file to use the same primary key as used in the gene sets file (GMT file). You have the following options:
  • Use the GSEA desktop application: GSEA / Tools / Collapse Dataset

  • Run this Python script collapse_ExpressionMatrix.py using the Chip platform file that was used by GSEA.

RNK (GSEA file type)

• RNK file is completely different from the GCT or TXT file. It represents a ranked list of genes containing only gene name and a rank or score.

• The first line contains column headings
  • (For example:) Gene Name (--tab--) Rank Name
• Each line of RNK file contains a:
  • name (--tab--) rank OR score

Additional Information on GSEA File Formats can be found here

TXT

• Basic file representing expression values for an experiment.
• The first line consists of column headings.
  • name (--tab--) description (--tab--) sample1 name (--tab--) sample2 name ...
• Each line of the expression file contains a:
  • name (--tab--) description (--tab--) followed by a list of tab delimited expression values.

Enrichment Results files

GSEA result files

• For each analysis GSEA produces two output files. One representing the enriched genesets in phenotype A and the other representing the enriched genesets in phenotype B.
• These files are usually named "gsea_report_for_phenotypeA.Gsea.########.xls" and "gsea_report_for_phenotypeB.Gsea.########.xls"
• The files should be loaded in as is and require no pre-processing.
• There is no need to worry about which Enrichment Results Text box to put the two files. The phenotype is specified by the sign of the ES score and is computed internally by the program.

Additional Information on GSEA File Formats can be found here

Generic results files

• The generic results file is a tab delimited file with enriched gene-sets and their corresponding p-values (and optionally, FDR corrections)
• The Generic Enrichment Results file needs:
  • gene-set ID (must match the gene-set ID in the GMT file),
  • gene-set name or description,
  • p-value,
  • FDR correction value
  • Phenotype: +1 or -1, to identify enrichment in up- and down-regulation, or, more in general, in either of the two phenotypes being compared in the two-class analysis
    • +1 maps to red
    • -1 maps to blue
  • gene list separated by commas

Notes:

1. description and FDR columns can have empty or NA values, but the column and the column header must exist
2. if no value is provided under phenotype, Enrichment Map will assume there is only one phenotype, and will map enrichment p-values to red

See here for examples

DAVID Enrichment Result File

• Available only in v1.0 or higher

• When using DAVID as the analysis type there is no requirement to enter either a gmt file or an expression file. Both are options if the user wishes to add them to the analysis.

• The DAVID Enrichment Result File is a file generated by the DAVID Functional Annotation Chart Report and consists of the following fields: Important: Make sure you are using CHART Report and NOT a Clustered Report

  • Category (DAVID category, i.e. Interpro, sp_pir_keywords, ...)
  • Term - Gene set name
  • Count - number of genes associated with this gene set
  • Percentage (gene associated with this gene set/total number of query genes)
  • P-value - modified Fisher Exact P-value
  • Genes - the list of genes from your query set that are annotated to this gene set.
  • List Total - number of genes in your query list mapped to any gene set in this ontology
  • Pop Hits - number of genes annotated to this gene set on the background list
  • Pop Total - number of genes on the background list mapped to any gene set in this ontology.
  • Fold enrichment
  • Bonferroni
  • Benjamini
  • FDR

Notes:

1. The DAVID option expects a file as generated by the DAVID web interface.

2. DISCLAIMER : In the absence of a gmt gene sets are constructed based on the field Genes in the DAVID output. This only considers the genes entered in your query set and not the genes in your background set. This will drastically affect the amount of overlap you see in the resulting Enrichment Map.

See here for tutorial on how to generate David output files for Enrichment maps

BiNGO Enrichment Result File

• Available only in v1.2 or higher

• When using BiNGO as the analysis type there is no requirement to enter either a gmt file or an expression file. Both are options if the user wishes to add them to the analysis.

• The BiNGO Enrichment Result File is a file generated by the BiNGO cytoscape plugin and consists of the following fields: Important: When running BiNGO make sure to check off "Check Box for saving data"

  • The first 20 lines of BiNGO output file list parameters used for the analysis and are ignored by the Enrichment map plugin

  • GO-ID - Gene set name
  • p-value - hypergeometric or binomial Exact P-value
  • corr p-value - corrected p-value
  • x - number of genes in your query list mapped to this gene-set
  • n - number of genes in the background list mapped to this gene-set
  • X - number of genes annotated to this gene set on the background list
  • N - number of genes on the background list mapped to any gene set in this ontology.
  • Description - gene list description
  • Genes - the list of genes from your query set that are annotated to this gene set.

Notes:

1. The BiNGO option expects a file as generated by the BiNGO Cytsocape Plugin.

2. DISCLAIMER : In the absence of a gmt gene sets are constructed based on the field Genes in the BiNGO output. This only considers the genes entered in your query set and not the genes in your background set. This will drastically affect the amount of overlap you see in the resulting Enrichment Map.

See here for tutorial on how to generate Bingo output files for Enrichment maps

RPT files

• A special trick for GSEA results, in any GSEA analysis an rpt file is created that specifies the location of all files (including the gmt, gct, results files, phenotype specification, and rank files).
• Any of the Fields under the dataset tab (Expression, Enrichment Results 1 or Enrichment Results 2) will accept an rpt file and populate GMT, Expression, Enrichment Results 1, Enrichment Results 2, Phenotypes, and Ranks the values for that dataset.
• A second rpt file can be loaded for dataset 2. It will give you a warning if the GMT file specified is different than the one specified in dataset 1. You will have the choice to use the GMT for data set 1, data set 2 or abort the second rpt load.
• An rpt file is a text file with following information (parameters surrounded by " ' ' '" are those that EM uses):

                '''producer_class'''    xtools.gsea.Gsea
                '''producer_timestamp'''        1367261057110
                param   collapse        false
                param   '''cls'''       WHOLE_PATH_TO_FILE/EM_EstrogenMCF7_TestData/ES_NT.cls#ES24_versus_NT24
                param   plot_top_x      20
                param   norm    meandiv
                param   save_rnd_lists  false
                param   median  false
                param   num     100
                param   scoring_scheme  weighted
                param   make_sets       true
                param   mode    Max_probe
                param   '''gmx'''       WHOLE_PATH_TO_FILE/EM_EstrogenMCF7_TestData/Human_GO_AllPathways_no_GO_iea_April_15_2013_symbol.gmt
                param   gui     false
                param   metric  Signal2Noise
                param   '''rpt_label''' ES24vsNT24
                param   help    false
                param   order   descending
                param   '''out'''       WHOLE_PATH_TO_FILE/EM_EstrogenMCF7_TestData
                param   permute gene_set
                param   rnd_type        no_balance
                param   set_min 15
                param   include_only_symbols    true
                param   sort    real
                param   rnd_seed        timestamp
                param   nperm   1000
                param   zip_report      false
                param   set_max 500
                param   '''res'''       WHOLE_PATH_TO_FILE/EM_EstrogenMCF7_TestData/MCF7_ExprMx_v2_names.gct

                file    WHOLE_PATH_TO_FILE/EM_EstrogenMCF7_TestData/ES24vsNT24.Gsea.1367261057110/index.html

• Parameters used by EM and their meaning:

  1. producer_class - can be xtools.gsea.Gsea or xtools.gsea.GseaPreranked

     • if xtools.gsea.Gsea:

       • get expression file from res parameter in rpt
       • get phenotype information from cls parameter in rot

     • if xtools.gsea.GseaPreranked:

       • No expression file
       • use rnk as the expression file from rnk parameter in rot
       • set phenotypes to na_pos and na_neg.
       • NOTE: if you want to make using an rpt file easier for GSEAPreranked there are two additional parameters you can add to your rpt file manually that the rpt function will recognize.

         • To do less manual work while creating Enrichment Maps from pre-ranked GSEA, add the following optional parameters to your rpt file:
                           param(--tab--)phenotypes(--tab--){phenotype1}_versus_{phenotype2}
                           param(--tab--)expressionMatrix(--tab--){path_to_GCT_or_TXT_formated_expression_matrix} 

  2. producer_timestamp - needed to find the directory with the results files
  3. cls - path to class/phenotype file with information regarding the phenotypes:
     • path/classfilename.cls#phenotype1_versus_phenotype2
     • EM get the path to the class file and also pulls the phenotype1 and phenotype2 from the above field
  4. gmx - path to gmt file
  5. rpt_label - name of analysis and name of directory that GSEA creates to hold the results. Used when constructing the path to the results directory.
  6. out - path to directory where GSEA will put the output directory. Used when constructing the path to the results directory.
  7. res - path to expression file.
• rpt Searches for the following results files:

Enrichment File 1 --> {out}(--File.separator--){rpt_label} + "." + {producer_class} + "." + {producer_timestamp}(--File.separator--) "gsea_report_for_" + phenotype1 + "_" + timestamp + ".xls"
Enrichment File 2 --> {out}(--File.separator--){rpt_label} + "." + {producer_class} + "." + {producer_timestamp}(--File.separator--) "gsea_report_for_" + phenotype2 + "_" + timestamp + ".xls"
Ranks File --> {out}(--File.separator--){rpt_label} + "." + {producer_class} + "." + {producer_timestamp}(--File.separator--) "ranked_gene_list_" + phenotype1 + "_versus_" + phenotype2 +"_" + timestamp + ".xls";      

• If the enrichments and rank files are not found in the above path then EM replaces the out directory with the path to the given rpt file and tries again.
• if you would like to create your own rpt file for your own analysis pipeline you can put your own values for the above used parameters.
• If your analysis only creates one enrichment file you can make a copy of enrichment file 1 in the path of enrichment file 2 with no consequences for EM running.

EDB File (GSEA file type)

• Contained in the GSEA results folder is an edb folder. In the edb folder there are the following files:
  1. results.edb
  2. gene_sets.gmt
  3. classfile.cls [Only in a GSEA analysis. Not in a GSEAPreranked analysis]
  4. rankfile.rnk
• If you specify the results.edb file in any of the Fields under the dataset tab (Expression, Enrichment Results 1 or Enrichment Results 2) the gmt and enrichment files fields will be automatically populated.

• If you want to associate an expression file with the analysis it needs to be loaded manually as described here.

  * NOTE: The gene_sets.gmt file contained in the edb directory is filtered according to the expression file.  If you are doing a two dataset analysis where the expression files are from different platforms or contain different sets of genes the edb gene_sets.gmt file can not be used as genes found in one analysis might be lacking in the other.  In this case use the original gmt file (prior to GSEA filtering) and EM will filter each the gene sets separately according to each dataset.

Advanced Settings - Additional Files

• For each dataset there are additional parameters that the user can set but are not required.
• The advanced parameters include:

  • Ranks file - file specifying the ranks of the genes in the analysis

    • This file has the format specified in the above section - gene (--tab--) rank or score. Seehere for details.

  • Phenotypes (phenotype1 versus phenotype2)

    • By default the phenotypes are set to Up and Down but in the advanced setting mode the user can change these to any desired text.

• All of these fields are populated when the user loads the input files using the rpt option.

Parameters

Node (Gene Set inclusion) Parameters

• Node specific parameters filter the gene sets included in the enrichment map
• For a gene set to be included in the enrichment map it needs to pass both p-value and q-value thresholds.

P-value

• All gene sets with a p-value with the specified threshold or below are included in the map.

FDR Q-value

• All gene sets with a q-value with the specified threshold or below are included in the map.
• Depending on the type of analysis the FDR Q-value used for filtering genesets by EM is different
  • For GSEA the FDR Q-value used is 8th column in the gsea_results file and is called "FDR q-val".
  • For Generic the FDR Q-value used is 4th column in the generic results file.
  • For David the FDR Q-value used is 12th column in the david results file and is called "Benjamini".
  • For Bingo the FDR Q-value used is 3rd column in the Bingo results file and is called "core p-value"

Edge (Gene Set relationship) Parameters

• An edge represents the degree of gene overlap that exists between two gene sets, A and B.
• Edge specific parameters control the number of edges that are created in the enrichment map.
• Only one coefficient type can be chosen to filter the edges

Jaccard Coefficient

                Jaccard Coefficient = [size of (A intersect B)] / [size of (A union B)]

Overlap Coefficient

                Overlap Coefficient = [size of (A intersect B)] / [size of (minimum( A , B))]

Combined Coefficient

• the combined coefficient is a merged version of the jacquard and overlap coefficients.
• the combined constant allows the user to modulate reciprocally the weights associated with the jacquard and overlap coefficients.
• When k = 0.5 the combined coefficient is the average between the jacquard and overlap.

                Jaccard Coefficient = [size of (A intersect B)] / [size of (A union B)]
                Overlap Coefficient = [size of (A intersect B)] / [size of (minimum( A , B))]
                
                Combined Constant = k

                Combined Coefficient = (k * Overlap) + ((1-k) * Jaccard)

Tips on Parameter Choice

P-value and FDR Thresholds

GSEA can be used with two different significance estimation settings: gene-set permutation and phenotype permutation. Gene-set permutation was used for Enrichment Map application examples.

Gene-set Permutation

Here are different sets of thresholds you may consider for gene-set permutation:

• Very permissive:

  • p-value < 0.05

  • FDR < 0.25

• Moderately permissive:

  • p-value < 0.01

  • FDR < 0.1

• Moderately conservative:

  • p-value < 0.005

  • FDR < 0.075

• Conservative:

  • p-value < 0.001

  • FDR < 0.05

For high quality, high coverage transcriptomic data, the number of enriched terms at the very conservative threshold is usually 100-250 when using gene-set permutation.

Phenotype Permutation

• Recommended:

  • p-value < 0.05

  • FDR < 0.25

In general, we recommend to use permissive thresholds only if your having a hard time finding any enriched terms.

Jaccard vs. Overlap Coefficient

• The Overlap Coefficient is recommended when relations are expected to occur between large-size and small-size gene-sets, as in the case of the Gene Ontology.
• The Jaccard Coefficient is recommended in the opposite case.
• When the gene-sets are about the same size, Jaccard is about the half of the Overlap Coefficient for gene-set pairs with a small intersection, whereas it is about the same as the Overlap Coefficient for gene-sets with large intersections.
• When using the Overlap Coefficient and the generated map has several large gene-sets excessively connected to many other gene-sets, we recommend switching to the Jaccard Coefficient.

Overlap Thresholds

• 0.5 is moderately conservative, and is recommended for most of the analyses.
• 0.3 is permissive, and might result in a messier map.

Jaccard Thresholds

• 0.5 is very conservative
• 0.25 is moderately conservative

Interfaces

The Input Panel

1. Analysis Type

   • There are two distinct types of Enrichment map analyses, GSEA or Generic.

     • GSEA - takes as inputs the output files created in a GSEA analysis. File formats are specific to files created by GSEA. The main difference between this and generic is the number and format of the Enrichment results files. GSEA analysis always has two enrichment results files, one for each of the phenotypes compared.

     • Generic - takes as inputs the same file formats as a GSEA analysis except the Enrichment results file is a different format and there is only one enrichment file. Generic File description

     • DAVID - (implemented in v1.0 and higher) has no gmt or expression file requirement and takes as input enrichment result file as produced by DAVID David Enrichment Result File description

2. Genesets - path to gmt file describing genesets. User can browse hard drive to find file by pressing ... button.

3. Dataset 1 - User can specify expression and enrichment files or alternatively, an rpt file which will populate all the fields in genesets,dataset # and advanced sections.

4. Advanced - Initially collapsed (expand by clicking on arrow head directly next to Advanced), users have the option of modifying the phenotype labels or loading gene rank files.

5. Parameters - User can specify p-value, fdr and overlap/jaccard cutoffs. Choosing Optimal parameter values

6. Actions - The user has three choices, Reset (clears input panel), Close (closes input panel), and Build Enrichment map (takes all parameters in panel and builds an Enrichment map)

The Data Panel

• The bottom (south) panel.

Expression Viewer

• There are two different types of Expression Viewers, each is represented as a separate tab in data panel: EM Overlap and EM Gene set. The only difference between the two expression viewers is the set of gene listed.

  1. EM Overlap Expression Viewer - shows the expression of genes in the overlap (intersection) of all the genesets selected

  2. EM Geneset Expression Viewer - shows the expression of genes of the union of all the genesets selected.

  3. Normalization

     • Data as is - represents the data as it was loaded
     • Row Normalize Data - for each value in a row of expression the mean of the row is subtracted followed by division by the row's standard deviation.
     • Log Transform Data - takes the log of each expression value

  4. Sorting

     • Hierarchical cluster - as computed using Pearson correlation of the entire expression set.
     • If rank files for the data sets are provided at input they will show up as 'Dataset 1 Ranking' and 'Dataset 2 Ranking' and by selecting them the user will be able to sort the expression accordingly
       • if an expression value does not have a corresponding rank in the ranking file its expression does not appear in the heatmap.

     • Add Ranking ... - allows user to upload an additional rank file (in the appropriate format,as outlined in Rank file descriptions). There is no limit on the number of rank files that are uploaded. The user is required to give a name to the rank file.

  5. Save Expression Set

     • The user can save the subset of expression values currently being viewed in the expression viewer as txt file.

  6. Export Expression Set (PDF)

     • The user can save the expression heatmap currently being viewed in the expression viewer as pdf file. Unfortunately the pdf version is not perfect. The column titles are at the bottom instead of the top of the heatmap. Due to limitations with the current library being used we are unable to fix this in cityscape 2.8.3 but hope to have a better pdf representation in the EM version for Cytoscape 3.0.

Node Attributes

• For each Enrichment map created the following attributes are created for each node:
  • EM#_Name - the gene set name

  • EM#_Formatted_name - a wrapped version of the gene set name so it is easy to visualize.
    

    • Note: This is the default label of the node but some users find it easier to arrange the network when the name is not wrapped. If this is the case in the vizmapper the user can switch the label mapping from EM#_formatted_name to EM#_name.

  • EM#_GS_DESCR - the gene set description (as specified in the second column of the gmt file)
  • EM#_Genes - the list of genes that are part of this gene set.
• Additionally there are attributes created for each dataset (a different set for each dataset if using two dataset mode):
  • EM#_pvalue_dataset(1 or 2) - Gene set p-value, as specified in GSEA enrichment result file.
  • EM#_qvalue_dataset(1 or 2) - Gene set q-value, as specified in GSEA enrichment result file.
  • EM#_Colouring_dataset(1 or 2) - Enrichment map parameter calculated using the formula 1-pvalue multiplied by the sign of the ES score (if using GSEA mode) or the phenotype (if using the Generic mode)
  • GSEA specific attributes (these attributes are not populated when creating an enrichment map using the generic mode)
    • EM#_ES_dataset(1 or 2) - Enrichment score, as specified in GSEA enrichment result file.
    • EM#_NS_dataset(1 or 2) - Normalized Enrichment score, as specified in GSEA enrichment result file.
    • EM#_fwer_dataset(1 or 2) - Family-wise error score, as specified in GSEA enrichment result file.

Edge Attributes

• For each Enrichment map created the following attributes are created for each edge:
  • EM#_Overlap_size - the number of genes associated with the overlap of the two genesets that this edge connects.
  • EM#_Overlap_genes - the names of the genes that are associated with the overlap of the two genesets that this edge connects.

  • EM#_similarity_coefficient - the calculated coefficient for this edge.
    

The Results Panel

• The right (east) panel

Parameters pane

• Reference panel containing legends, slider bars for the user to modify p-value and q-value cut-offs, parameters used for the analysis

1. Phenotype 1
2. Phenotype 2

3. P-value Cutoff tuner - allows you to adjust the p-value threshold used to filter the gene sets.

   • By moving the slider to the left you can decrease the p-value threshold causing nodes (and their edges) to be removed from the network.
   • Moving the slider back to the right will restore the nodes (and their edges).
   • You can NOT increase the p-value threshold above what you specified when you built the network.

4. Q-value Cutoff tuner - allows you to adjust the q-value threshold used to filter the gene sets.

   • By moving the slider to the left you can decrease the q-value threshold causing nodes (and their edges) to be removed from the network.
   • Moving the slider back to the right will restore the nodes (and their edges).
   • You can NOT increase the q-value threshold above what you specified when you built the network.

5. Similarity Cutoff tuner - allows you to adjust the similarity threshold used to filter the gene set overlaps (edges).

   • By moving the slider to the right you can increase the similarity threshold causing edges to be removed from the network.
   • Moving the slider back to the left will restore the edges.
   • You can NOT decrease the similarity threshold below what you specified when you built the network.
6. Button to launch index of GSEA results in a web browser.
7. List of parameters used to create the EM.

8. Heatmap Autofocus

   • selected by default

   • When you click on any node or edge in the network EM automatically updates the expression viewer and makes the focus of the Data panel the overlap expression viewer. When using other plugins in conjunction with EM this feature can get cumbersome.

   • To turn this off unselect "heatmap Autofocus".

9. Default Sorting order - in the expression viewer genes can be sorted by Hierarchical clustering, Ranks, Columns, or No sort. To set the default change selection.

10. Default Distance Metric - for hierarchical clustering there are three available distance metrics that can be used to compute distances between genes. By default this is set of pearson correlation. Update this parameter if you which to use one of the other distance metrics.

PostAnalysis Input Panel

To access Post Analysis go to the menu path: Apps > Enrichment Map > Load Post Analysis Panel.

There are currently two types of Post Analysis Available: Known Signature and Signature Discovery. The contents of the panel will change depending on the type of analysis chosen. Known signature mode calculates post analysis edges for a small subset of known gene-sets. Signature discovery mode allows for filtering of large set of potential signatures to help uncover most likely sets.

The result of running Post Analysis is a new node for each signature gene set (yellow triangle) and edges from the signature gene set to each existing gene set when the similarity passes the cutoff test.

A new visual style is also created and applied to the network after post analysis runs. This visual style is the same as the enrichment map style but with a few additions. Signature edges are pink, signature nodes are yellow triangles, and edge width mapping is calculated differently.

Known Signature

1. Post Analysis Type

   • Known Signature: Calculates the overlap between gene-sets of the current Enrichment Map and all the gene sets contained in the provided signature file.

2. Gene Sets

   • SigGMT: The gmt file with the signature-genesets. These will be compared against the gene-sets from the current Enrichment Map.

3. Edge Weight Parameters: Choose a method for generating an edge between a signature-geneset and an enrichment geneset. Described in detail below.

4. Actions: Reset (clears input panel), Close (closes input panel), and Run (takes all parameters in panel and performs the Post-Analysis)

Signature Discovery

1. Post Analysis Type

   • Signature Discovery: Calculates the overlap between gene-sets of the current Enrichment Map and the selected genesets.

2. Gene-Sets

   • The gmt file with the signature-genesets.
   • Filter: Genesets from the gmt file that do not pass the filter test will not be loaded.
   • Load Gene-Sets: Press after the gmt file and filter have been chosen to load the signature-genesets.

3. Available Signature Genesets: Once the genesets have been loaded this box will contain a list of all the genesets in the SigGMT file (that passed the filter).

   • To highlight more htan one geneset at at time hold the Shift, Command or Ctrl keys while clicking with the mouse.

4. Selected Signature Genesets: The analysis will be performed with all genesets in this list. Use the down- and up-buttons to move highlighted genesets from one list to the other.

5. Edge Weight Parameters: Choose a method for generating an edge between a signature-geneset and an enrichment geneset. Described in detail below.

6. Actions: Reset (clears input panel), Close (closes input panel), and Run (takes all parameters in panel and performs the Post-Analysis)

Edge Weight Parameters

1. Test: Select the type of statistical test to use for edge width.

2. Cutoff: Edges with a similarity value lower than the cutoff will not be created.

3. Data Set: If the enrichment map contains multiple data sets choose the one to use here.

4. Notes:

   • The results of the calculations will be available in the edge table after post analysis runs.

   • The edge “interaction type” will be sig.

   • The hypergeometric test is always calculated, even if it is not used for the cutoff. The results are made available in the edge table.

5. Available Tests

   • Hypergeometric Test is the probability (p-value) to find an overlap of k or more genes between a signature geneset and an enrichment geneset by chance.
     

     • 
       with:
       k (successes in the sample) : size of the Overlap,
       n (size of the sample) : size of the Signature geneset
       m (total number of successes) : size of the Enrichment Geneset
       N (total number of elements) : size of the union of all Enrichment Genesets

     • Advanced Hypergeometric Universe: Allows to choose the value for N. (GMT: all the genes in the original gmt file, Expression Set: number of genes in the expression set, Intersection: number of genes in the intersection of the gmt file and expression set, User Defined: manually enter a value).

   • Overlap has at least X genes

     • The number of genes in the overlap between the enrichment map gene set and the signature gene set must be at least X for the edge to be created.

   • Overlap is X percent of EM gs

     • The size of the overlap must be at least X percent of the size of the Enrichment Map gene set.

   • Overlap is X percent of Sig gs

     • The size of the overlap must be at least X percent of the size of the Signature gene set.

   • Mann-Whitney (Two-sided, one-sided greater, one-sided less)

     • Note: The Mann-Whitney test requires ranks. It will not be available if the enrichment map was created without ranks.
     • Calculates the p-value using the Mann-Whitney U test where the first sample is the ranks in the overlap and the second sample is all of the ranks in the expression set.

Edge Width

• When you create an Enrichment Map network a visual style is created. The default edge with property is a continuous mapping to the “similarity_coefficient” column. After running post-analysis the rules for calculating edge width become more complicated. Edge width for edges between enrichment sets are still based on the “similarity_coefficient” column, but edges between signature sets and enrichment sets are based on the statistical test used for cutoff. Currently cytoscape does not provide a visual mapping that is capable of “if-else” logic. In order to work around this limitation, the width of the edges is calculated by EnrichmentMap and put into a new column called “EM1_edge_width_formula”. Then the edge width property uses a continuous mapping to that column.

• Edge Width Dialog

  • In order to change the rules used to calculate edge width go to the menu path: Apps > EnrichmentMap > Post Analysis Edge Width.

  • Geneset Overlap: Set the end points of the contiuous mapping for edge width for edges between enrichment sets.

  • Signature Set: Set the edge width value for signature set edges that are less than cutoff/100, <= cutoff/10 and > cutoff/10.

  • Click OK to recalculate the values in the “EM1_edge_width_formula” column.

Additional Features

Launch Enrichment Map from the command line

• Requirements:
  1. Enrichment Map v1.3 or higher

  2. Commandtool App - available from Cytsocape App store. Documentation can be found here

  3. GSEA results in an edb directory
• command tool can be used from:
  1. the command line
     • java -Xmx1G -jar "{path_to_cytoscape_dir}\cytoscape.jar" -p "{path_to_plugin_dir}\plugins" -S "{path_to_script_file}"
  2. cytoscape command window

     • (Plugins->commandtool-->Command Window...)

  3. script file

     • (Plugins->commandtool-->Run Script...)

• Command Options

  enrichmentmap build: Build an enrichment map from GSEA results (in an edb directory)
    Arguments:
      [edbdir=value] --> REQUIRED
      [expressionfile=value] --> OPTIONAL
      [overlap=value] --> OPTIONAL
      [pvalue=value] --> OPTIONAL
      [qvalue=value] --> OPTIONAL
      [similaritymetric=value] --> OPTIONAL
      [combinedconstant=value] --> OPTIONAL

• Example Command (for command window)

enrichmentmap build edbdir="{path_to_edb_directory}" pvalue=0.01 qvalue=0.1 overlap=0.5 similaritymetric="jaccard"
                         expressionfile="{path_to_expression_file}"

Distinct Species or Platform Analysis

Bulk Enrichment Map Build

Calculate Gene set relationships

• To analyze the relationships that exists between genesets in the absence of an enrichment analysis an Enrichment map can be built with just the gene set definition file (gmt file)

• In the input panel specify only a gmt file and click on build.

• DISCLAIMER: This task requires a lot of memory. In a normal enrichment analysis we compute similarities only for the gene sets that pass the thresholds in addition to constraining the genes of the gene sets by the given expression set which drastically decreases the computations of similarity required. The smaller the gmt file the less memory required.

GSEA Leading Edge Functionality

• For every gene set that is tested for significance using GSEA there is a set of proteins in that gene set defined as the Leading Edge. According to GSEA the leading edge is:

"the subset of members that contribute most to the ES. For a positive ES, the leading edge subset is the set of members that appear in the ranked list prior to the peak score. For a negative ES, it is the set of members that appear subsequent to the peak score."

• In essence, the leading edge is the set of genes that contribute most to the enrichment of the gene set.

• For Enrichment Map, leading edge information is extracted from the gsea enrichment results files from the column denoted as Rank at Max. Rank at max is the rank of the gene where the ES score has the maximal value, i.e. the peak ES score. Everything with a better rank than the rank at max is part of the leading edge set.

1. To access GSEA leading edge information click on an individual Node. Leading edge information is currently only available when looking at a single gene set.

2. In the Data Panel the expression profile for the selected gene set should appear in the EM GenesetExpression viewer tab.

3. Change the Normalization to your desired metric.

4. Change the Sorting method to GSEARanking.

5. Genes part of the leading edge are highlighted in Yellow.

Customizing Defaults with Cytoscape Properties

The Enrichment Map Plugin evaluates a number of Cytoscape Properties with which a user can define some customized default values.
These can be added and changed with the Cytoscape Preferences Editor (Edit / Preferences / Properties...) or by directly editing the file cytoscape.props within the .cytoscape folder in the User's HOME directory.

Supported Cytoscape Properties are:

EnrichmentMap.default_pvalue

Default P-value cutoff for Building Enrichment Maps

Default Value: 0.05

valid Values: float >0.0, <1.0

EnrichmentMap.default_qvalue

Default Q-value cutoff for Building Enrichment Maps

Default Value: 0.25

valid Values: float >0.0, <1.0

EnrichmentMap.default_overlap

Default Overlap coefficient cutoff for Building Enrichment Maps

Default Value: 0.50

valid Values: float >0.0, <1.0

EnrichmentMap.default_jaccard

Default Jaccard coefficient cutoff for Building Enrichment Maps

Default Value: 0.25

valid Values: float >0.0, <1.0

EnrichmentMap.default_overlap_metric

Default choice of similarity metric for Building Enrichment Maps

Default Value: Jaccard

valid Values: Jaccard, Overlap

EnrichmentMap.default_sort_method

Set the default sorting in the legend/parameters panel to Hierarchical Clustering,

• Ranks (default the first rank file, if no ranks then it is no sort), Column (default is the first column) or no sort.

Default Value: Hierarchical Cluster

valid Values: Hierarchical Cluster, Ranks, Columns, No Sort

EnrichmentMap.hieracical_clusteting_theshold

Threshold for the maximum number of Genes before a dialogue opens to confirm if clustering should be performed.

Default Value: 1000

valid Values: Integer

nodelinkouturl.MSigDb.GSEA Gene sets

LinkOut URL for MSigDb.GESA Gene sets.

Default Value: http://www.broad.mit.edu/gsea/msigdb/cards/%ID%.html

valid Values: URL

EnrichmentMap.disable_heatmap_autofocus

Flag to override the automatic focus on the Heatmap once a Node or Edge is selected.

Default Value: FALSE

valid Values: TRUE, FALSE

FAQ

• Q: One of my enrichment map clusters has so much overlapping, I'm having a hard time interpreting it.
  A: Quickest solutions:

  1. Menu: Layout / Scale to expand the sub-network of interest (it will reduce clutter, but may take a lot of space)
  2. Results Panel: reduce the Q-value cutoff (it will make the sub-network less crowded, at the expense of the less relevant gene-sets)
  3. Clone the sub-network into a new network and use organic layout (organic layout has less clutter than force directed)

  4. Use the Software/WordCloudPlugin to summarize the node labels

  5. Make an enrichment map only of that sub-network using the Jaccard coefficient
     • this needs some scripting: you have to restrict the GMT to the gene-sets in the sub-network and make a new enrichment map selecting the Jaccard coefficient (instead of Overlap).
     • Jaccard usually works better with super-connected sub-networks, i.e. the ones arising when you have lots of GO-derived redundancy