Enrichment Map Plugin Download Page

Brief Description

Enrichment Map is a Cytoscape plugin for functional enrichment visualization. Enrichment results have to be generated outside Enrichment Map, using any of the available methods. Gene-sets, such as pathways and Gene Ontology terms, are organized into a network (i.e. the "enrichment map"). In this way, mutually overlapping gene-sets cluster together, making interpretation easier. Enrichment Map also enables the comparison of two different enrichment results in the same map.

Follow this link for more details and an analysis example.

Plugin Download

1. Updated at 07/06/2011 - Release 1.2 : Compatible with cytoscape 2.6,2.7,2.8

   • Enrichment Map Plugin (v1.2 Release)
     Download the zipped file, move it to the Cytoscape plugin directory and unzip it.
     

     Distributions with source code:
     

   • Enrichment Map Plugin with source code (v1.2 Release)

   • Enrichment Map Plugin with source code, tar and gzipped (v1.2 Release)

   • Added Features and Release Notes

2. Updated at 02/7/2011 - Release 1.1: Compatible with cytoscape 2.6,2.7,2.8

   • Enrichment Map Plugin (v1.1 Release)
     Download the zipped file, move it to the Cytoscape plugin directory and unzip it.
     

     Distributions with source code:
     

   • Enrichment Map Plugin with source code (v1.1 Release)

   • Enrichment Map Plugin with source code, tar and gzipped (v1.1 Release)

Development Versions

1. Updated at 05/05/2013 - Release 1.3 - DEVELOPMENT Release : Compatible with cytoscape 2.6,2.7,2.8

   • Added bug fix with missing leading edge on reloaded sessions files for down regulated gene sets.
   • Added command tool support
   • Added EDB support

   • Enrichment Map Plugin (v1.3 DEV)
     Download the zipped file, move it to the Cytoscape plugin directory and unzip it.
     

2. Updated at 08/05/2011 - Release 1.3 - DEVELOPMENT Release : Compatible with cytoscape 2.6,2.7,2.8

   • Added bug fix with missing leading edge on reloaded sessions files for down regulated gene sets.

   • Enrichment Map Plugin (v1.3 DEV)
     Download the zipped file, move it to the Cytoscape plugin directory and unzip it.
     

     Distributions with source code:
     

   • Enrichment Map Plugin with source code (v1.3 DEV)

   • Enrichment Map Plugin with source code, tar and gzipped (v1.3 DEV)

Tutorials and Examples

data used for the tutorials: Estradiol-treated MCF7 cells, 12 and 24hrs (Gene Expression Omnibus: GSE11352)

• GSEA format:

  • files: EM_EstrogenMCF7_TestData.zip

  • Guided tutorial to load the files into Enrichment Map

• Generic format:

  • files: EM_EstrogenMCF7_TestData_Generic.zip

  • note: the results should be the same as GSEA

• DAVID format:

  • files: EM_David_testData.zip

  • Guided DAVID Tutorial to load the files into Enrichment Map

• BiNGO format:

  • files: EM_Bingo_testData.zip

  • Guided BiNGO Tutorial to load the files into Enrichment Map

• Query-set analysis (aka Post-analysis)

  • Estrogen targets (Lin CY et al 2007, Plos Genetics; PMID: 17542648)

User Guide

Link to User Guide (and FAQ)

Gene-sets for Enrichment Analysis

• we generate gene-set files for enrichment analysis (e.g. GSEA) by querying public resources such as Gene Ontology and KEGG, and using Entrez-Gene ID for genes

  download page

• how to convert DAVID gene-sets to GMT: R script

Publications

Cite EM

• Enrichment Map: A Network-Based Method for Gene-Set Enrichment Visualization and Interpretation
  Merico D, Isserlin R, Stueker O, Emili A, Bader GD
  PLoS One. 2010 Nov 15;5(11):e13984
  PubMed Abstract -PDF
  

Examples of Use

• Functional impact of global rare copy number variation in autism spectrum disorders.
  Pinto D, Pagnamenta AT, Klei L, Anney R, Merico D, Regan R, Conroy J, Magalhaes TR, Correia C, Abrahams BS, Almeida J, Bacchelli E, Bader GD, et al.
  Nature. 2010 Jun 9 (Epub ahead of print)
  PubMed Abstract - PDF
  Nature Blogs - In the news (The Globe and Mail)
  

• Pathway analysis of dilated cardiomyopathy using global proteomic profiling and enrichment maps
  Isserlin R, Merico D, Alikhani-Koupaei R, Gramolini A, Bader GD, Emili A.
  Proteomics 2010, March 10(6):1316-27
  Pubmed Abstract - PDF
  

Papers Citing Enrichment Map

• Citations in Pubmed Central

• Pathway analysis of expression data: deciphering functional building blocks of complex diseases.
  Emmert-Streib F, Glazko GV.
  PLoS Comput Biol. 2011 May;7(5):e1002053.
  PubMed

• Inflammasome is a central player in the induction of obesity and insulin resistance.
  Stienstra R, van Diepen JA, Tack CJ, Zaki MH, van de Veerdonk FL, Perera D, Neale GA, Hooiveld GJ, Hijmans A, Vroegrijk I, van den Berg S, Romijn J, Rensen PC, Joosten LA, Netea MG, Kanneganti TD.
  Proc Natl Acad Sci U S A. 2011 Aug 29.
  PubMed

• Delineation of Two Clinically and Molecularly Distinct Subgroups of Posterior Fossa Ependymoma
  Witt H, Mack SC, Ryzhova M, Bender S, Sill M, Isserlin R, Benner A, Hielscher T, Milde T, Remke M, Jones DTW, Northcott PA, Garzia L, Bertrand KC, Wittmann A, Yao Y, Roberts SS, Massimi L, Van Meter T, Weiss WA, Gupta N, Grajkowska W, Lach B, Cho YJ, von Deimling A, Kulozik AE, Witt O, Bader GD, Hawkins CE, Tabori U, Guha A, Rutka JT, Lichter P, Korshunov A, Taylor MD, Pfister SM
  Cancer Cell, Volume 20, Issue 2, 143-157, 16 August 2011
  PubMed Abstract - PDF
  

Report a Bug or a Problem

1. please make sure you don't have formatting issues (have a look at the User Guide (and FAQ))

   • if you are still not sure how to handle formats,
   • or you don't know what's the best suitable analysis for you,
   • please send an email to: daniele[AT]merico[DOT]gmail.com
2. please check what's your
   • plugin version and build (from the Cytoscape menu / Plugins / Enrichment Map / About)
   • Cytoscape version (from the Cytoscape menu / Cytoscape)
   • Operating System (e.g. Windows Vista)
   and send your bug report to ruth[DOT]isserlin[AT]utoronto.ca

3. For Feature requests please send to enrichmentmap-dev@googlegroups.com