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| == Table of Contents == [[TableOfContents()]] |
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| * Predict specificity of peptide recognition domain from the primary amino acid sequence. | * Computationally predict specificity of peptide recognition domain from the primary amino acid sequences |
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| == Background == * [wiki:/PDZ PDZ Domains] * [wiki:/MachineLearning Machine Learning] |
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| * [wiki:/Strategy Strategy] == Ideas == * [wiki:/Ideas Ideas] == Data == * [wiki:/PDZData PDZ Data] == Experiments == * [wiki:/Experiments Experiments and Results] |
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| * [wiki:/Log Status Log] | * [wiki:/Log Status] |
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| == Tasks == | ## == Tasks == ## ## 1. --(Learn SVN, Brain code (!ResidueResidueCorrelation))-- ## 1. Literature review related to domain specificity (background activity), PDZ domains (from Ioana's project) ## 1. --(Run !ResidueResidue correlation analysis on PDZ domain data: 1-1 version + try others e.g. 1-2 (Requires: PDZ profiles from Gary))-- ## 1. MSA subproject ## 1. --(Learn basics of multiple sequence alignment (Baxevanis, chapter 12))-- ## 1. Find and evaluate MSA algorithms (compare notes with Stacy) + evaluate Superfamily, PFAM databases of protein family alignments ## 1. Try different multiple sequence alignment algorithms (MSA) on the PDZ domain sequences to see if they affect the correlation results. ## 1. Benchmark/validate correlation subproject ## 1. We know H (PDZ), T @-2 (peptide) correlation ## 1. Look at structures (e.g. 1N7T and 1BE9) to see if correlated residues/positions are close to each other and compatible (physicochemically). We need to focus on ## PDZ structures that have bound peptides (search in PDB) ## 1. Build set of known true and false correlations for use in evaluating prediction algorithm (Note: also ask Dev Sidhu, when available). See [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=10871264 Baldi et al. review] ## 1. Amino acid group subproject ## 1. Learn about amino acid groups ## 1. Define an initial aa grouping (reasonable grouping from Levy paper) ## 1. Add new feature to !ResidueResidueCorrelation class so it considers grouping + run on PDZ data. This involves implementing the groups as a reduced alphabet (amino acids in a group are considered equivalent) ## 1. Try all groupings to see how it affects the results (from Levy paper) ## 1. See if we can incorporate aa similarity defined by substitution matrix approach (e.g. BLOSUM, PAM, GONNET) into our method, instead of grouping ## 1. Similarly, evaluate aa similarity defined by factor analysis (Atchley et al paper) ## 1. Think about new PDZ domain features that can be used for prediction. |
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| 1. Learn SVN, Brain code (!ResidueResidueCorrelation) 1. Literature review related to domain specificity (background activity), PDZ domains (from Ioana's project) 1. Run !ResidueResidue correlation analysis on PDZ domain data: 1-1 version + try others e.g. 1-2 (Requires: PDZ profiles from Gary) 1. MSA subproject 1. Learn basics of multiple sequence alignment (Baxevanis, chapter 12) 1. Find and evaluate MSA algorithms (compare notes with Stacy) + evaluate Superfamily, PFAM databases of protein family alignments 1. Try different multiple sequence alignment algorithms (MSA) on the PDZ domain sequences to see if they affect the correlation results. 1. Benchmark/validate correlation subproject 1. We know H (PDZ), T @-2 (peptide) correlation 1. Look at structures (e.g. 1N7T and 1BE9) to see if correlated residues/positions are close to each other and compatible (physicochemically) 1. Build set of known true and false correlations for use in evaluating prediction algorithm (Note: also ask Dev Sidhu, when available). See [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=10871264 Baldi et al. review] 1. Amino acid group subproject 1. Learn about amino acid groups 1. Define an initial aa grouping (reasonable grouping from Levy paper) 1. Add new feature to !ResidueResidueCorrelation class so it considers grouping + run on PDZ data 1. Try all groupings to see how it affects the results (from Levy paper) 1. See if we can incorporate aa similarity defined by weight matrix approach (e.g. BLOSUM, PAM, GONNET) into our method, instead of grouping 1. Similarly, evaluate aa similarity defined by factor analysis (Atchley et al paper) 1. Think about new PDZ domain features that can be used for prediction. |
## == Ideas == ## * [wiki:/MachineLearning Machine Learning Page] ## * With current correlation counting calculation, Weight calculation by how many peptides are in the peptides file (i.e. normalize the correlation calculation in some way) ## * Build tools to help interpret correlations in the context of multiple sequence alignments (and later structures). ## * Use of structural data (PDZ domain structures) (may require homology modeling) ## * Use of machine learning methods (SVM for classification and boosting decision tree for interpretable learning model) ## * Analysis of correlation within domain and peptide (inter-residue correlation) maybe correspondence analysis ## * Analysis of SNPs and how they affect domain binding (including correlations between SNPs) ## * Define the binding site of the PDZ domain based on phage display data. Given that identical binding sites between two PDZ domains should correspond to identical ## binding specificities, find the set of PDZ domain sites that correlate perfectly with binding specificity. |
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| == Ideas == * Use of structural data (PDZ domain structures) (may require homology modeling) * Use of machine learning methods (SVM for classification and boosting decision tree for interpretable learning model) * Analysis of correlation within domain and peptide (inter-residue correlation) maybe correspondence analysis |
## == Courses == ## === Biology === ## * [http://bio250y.chass.utoronto.ca/ BIO250] - Cell and Molecular Biology ## * Classes: Tues/Thurs - 1-2 PM (Convocation Hall) OR Mon - 6-8 PM (MC 102-Mechanical Engineering Building) ## * Textbook: [http://www.amazon.com/Molecular-Biology-Fourth-Bruce-Alberts/dp/0815332181/ref=pd_sim_b_1/105-5132391-0345258?ie=UTF8&qid=1188913552&sr=1-4 Molecular Biology of the Cell 4th Ed.] Alberts et al. ## === Protein Structure === ## * BCH340H1 - Proteins: from Structure to Proteomics ## * Classes: Winter 2008 ## * Textbook: ? ## * Previous Course Web Pages: ## * [http://arrhenius.med.utoronto.ca/~chan/bch340h04-outline.html 2004 Chan] ## * [http://xtal.uhnres.utoronto.ca/prive/BCH340/ 2006 Prive] ## === Machine Learning === ## * CSC2515 - Machine Learning ## * Previous Course Web Pages: ## * [http://www.cs.toronto.edu/~roweis/csc2515/ 2003-2006 Roweis] ## == Committee Meetings == ## * [wiki:/Meeting Notes] == Tools/Resources == * [wiki:/ToolsResources Tools and Resources] == Reading Notes == * [wiki:/ShirleyHui/MBCReadings Molecular Biology of the Cell] * [wiki:/ShirleyHui/MBCReadings Protein-protein Interaction Detection] * Support Vector Machines == Related Literature == * [http://www.connotea.org/rss/user/s2hui?download=view Literature List on Connotea] * [http://www.baderlab.org/DomainSpecificityPredictionProject/Reading Molecular Biology of the Cell] |
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| == Tools/Resources == === Databases === * [http://www.ensembl.org/ Ensembl] * Software system which produces and maintains automatic annotation on selected eukaryotic genomes. * [http://www.ebi.ac.uk/interpro/ InterPro] * Database of protein families, domains and functional sites in which identifiable features found in known proteins can be applied to unknown protein sequences. * [http://www.biomart.org/ BioMart] * Query-oriented data management system that simplifies the task of creation and maintenance of advanced query interfaces backed by a relational database. It is particularly suited for providing the 'data mining' like searches of complex descriptive (e.g. biological) data. === Sequence Alignment === ==== Multiple ==== * [http://www.drive5.com/muscle/ Muscle] * MUltiple Sequence Comparison by Log-Expectation. * [http://www.ebi.ac.uk/clustalw/ ClustalW] === Viewers === * [http://www.jalview.org/ JalView] * Multiple alignment viewer/editor written in Java == Background Literature == === More General === * The Human and Mouse Complement of SH2 Domain Proteins-Establishing the Boundaries of Phosphotyrosine Signaling, Liu BA, Jablonowski K, Raina M, Arce M, Pawson T and Nash PD, Mol Cell, 2006 Jun 23; 22(6):851-68. * attachment:Human_mouse_complement_of_SH2_domain_proteins_Liu_2006.pdf * Domains, motifs, and scaffolds: the role of modular interactions in the evolution and wiring of cell signaling circuits, Bhattacharyya RP, Remenyi A, Yeh BJ, Lim WA., Annu Rev Biochem. 2006;75:655-80. * attachment:Domains_motifs_scaffolds_Bhattacharyya_et_al_2006.pdf * The Structure and Function of Proline Recognition Domains, Zarrinpar A, Bhattacharyya RP, Lim WA., Sci STKE. 2003 Apr 22;2003(179):RE8. * attachment:Structure_Function_Pro_Recog_Domains_Zarrinpar_et_al_2003.pdf === Specificity Prediction/Inference === * Ab initio prediction of transcription factor targets using structural knowledge, Kaplan T, Friedman N, Margalit H, PLoS Comput Biol. 2005 Jun;1(1):e1. Epub 2005 Jun 24. * attachment:Ab_Initio_Prediction_Transcription_Factor_Targets_Using_Structural_Knowlegde_Kaplan_2005.pdf * Specificity and robustness in transcription control networks, Sengupta AM, Djordjevic M, Shraiman BI, Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2072-7. * attachment:Specificity_robustness_transcription_control_networks_Sengupta_2002.pdf * Can we infer peptide recognition specificity mediated by SH3 domains?, Cesareni G, Panni S, Nardelli G, Castagnoli L., FEBS Lett. 2002 Feb 20;513(1):38-44. * attachment:Can_we_infer_PR_specificity_med_by_SH3_Cesareni_et_al_2002.pdf === Amino Acid Alphabets === * Simplifying amino acid alphabets by means of a branch and bound algorithm and substitution matrices, Cannata N, Toppo S, Romualdi C, Valle G, Bioinformatics. 2002 Aug;18(8):1102-8. * attachment:Simplifying_AA_alphabets_branch_bound_substit_matrices_Cannata_2002.pdf * Simplified amino acid alphabets for protein fold recognition and implications for folding, Murphy LR, Wallqvist A, Levy RM, Protein Eng. 2000 Mar;13(3):149-52. * attachment:Simplified_AA_alphabets_Murphy_2000.pdf * Iterative sequence/secondary structure search for protein homologs: comparison with amino acid sequence alignments and application to fold recognition in genome databases, Wallqvist A, Fukunishi Y, Murphy LR, Fadel A, Levy RM, Bioinformatics. 2000 Nov;16(11):988-1002. * attachment:Iterative_structure_search_for_protein_homologs_Wallqvist_2000.pdf === PDZ Related === * PDZ domains-glue and guide., van Ham M, Hendriks W., Mol Biol Rep. 2003 Jun;30(2):69-82. * attachment:PDZ_Domains_Glue_and_Guide_2003.pdf * PDZ domains: structural modules for protein complex assembly., Hung AY, Sheng M., J Biol Chem. 2002 Feb 22;277(8):5699-702. Epub 2001 Dec 10. * attachment:PDZ_Domains_Structural_Modules_2001.pdf |
Table of Contents
Goals
- Computationally predict specificity of peptide recognition domain from the primary amino acid sequences
- Analyze PDZ, WW and then SH3 domains
Background
- [wiki:/PDZ PDZ Domains]
[wiki:/MachineLearning Machine Learning]
Strategy
- [wiki:/Strategy Strategy]
Ideas
- [wiki:/Ideas Ideas]
Data
- [wiki:/PDZData PDZ Data]
Experiments
- [wiki:/Experiments Experiments and Results]
Status
- [wiki:/Log Status]
Tools/Resources
[wiki:/ToolsResources Tools and Resources]
Reading Notes
- [wiki:/ShirleyHui/MBCReadings Molecular Biology of the Cell]
- [wiki:/ShirleyHui/MBCReadings Protein-protein Interaction Detection]
- Support Vector Machines
Related Literature
[http://www.connotea.org/rss/user/s2hui?download=view Literature List on Connotea]
[http://www.baderlab.org/DomainSpecificityPredictionProject/Reading Molecular Biology of the Cell]
Team
- Shirley Hui
- Gary Bader